Callus Induction from Zygotic Embryos of Coconut MATAG F2

The effect of 2,4-D (in concentrations of 5.0, 10.0, 20.0 and 40.0 mg/L) applied in combination with BAP (1.0, 10.0 and 20.0 mg/L) on callus induction in coconut MATAG F2 zygotic embryos cultured on Murashige and Skoog (MS) medium was investigated. The effects of IBA, TDZ and NAA combined with 2,4-D were also tested. The best callus formation (20%) was obtained on MS medium supplemented with 2,4-D at 10.0 mg/L. The induced calli were yellowish in colour and structurally compact. Different portions of the zygotic embryo were also compared for callus induction when used as explants and cultured on MS medium supplemented 10.0 mg/L 2,4-D. The incidence of callus formation (up to 83%) was highest from the middle portion of the embryos.