Isolation of Bacterial Colonies from House Fly Mouth Parts

Housefly (Musca Domestica Li.,1758) is a mechanical vector of pathogens spreading infectious diseases such as Cholera, Shigellosis, Salmonellosis and skin infections in human and domestic animals. The main objective is to isolate the bacteria from housefly mouthparts. The houseflies were collected from different places (sources) such as dairy farms, Fruit stalls, Fish markets and butcher shops located in the surrounding region. The mouth part of the housefly is dissected and it is dipped in saline water. This infested Saline water is diluted at different concentrations using the serial dilution method and inoculated in the agar plates using the pour plate method. Three different Agar such as Pseudomonas, EMBL and Nutrient agar are used. From the culture obtained, bacterial colony count is made for each and the values are compared with bacterial colonies obtained from different sources. Morphological variation of bacterial colony is also observed. Further Gram staining Procedures are done to observe the varied presence of Gram-positive and Gram-negative bacteria