HEMOGLOBIN ESTIMATION BY USING COPPER SULPHATE METHOD

The use of the copper sulphate method is based on the idea that when a drop of whole blood is allowed to fall into a solution of copper sulphate, an insoluble copper proteinate is created. Depending on how this one drop of blood behaves in the solution, the donor's blood will either be rejected or accepted. This procedure of Hemoglobin Estimation holds a great relevance in blood banks as it is cheap and gives fast result.

Purpose: Hemoglobin estimation is done for every donor who presents himself / herself for blood donation to select healthy blood donors.

Scope: Selection of healthy blood donors provides better post transfusion hemoglobin increment resulting in better oxygen carrying capacity as well as prevents donor to be anemic after donation

Responsibility:
i. Check the quantity of copper sulfate stock solution every day at 5:00 pm, if stock is insufficient; prepare stock solution for next day. Preparation of fresh copper sulfate working solution every day in the morning.
ii. Standardization of copper sulfate solution
iii. Take 50 ml of solution and change after performing 20 –25 tests.
iv. Dispose used solution as biohazardous material because of blood in the container.
v. In case of doubtful result confirm by automated sysmex-1000 prior to accepting or deferring the donor.
Responsible person: Designated technician and / or Nursing staff.