Somatic Embryogenesis of Cork Oak (Quercus suber L.): Influence of Culture Medium on the Secondary Somatic Regeneration

In this chapter, we present the technique of somatic embryogenesis applied to cork oak (Quercus
suber L). Also, we will show how the effectiveness of this technique can be improved based on the
phase of secondary somatic embryogenesis. Specially, we try to demonstrate the effect of the culture
medium composition on the massive multiplication of the embryos by secondary somatic
embryogenesis. To achieve this aim, several factors affecting the proliferation and maturation of SSE,
such as the basic formulation of culture medium, were evaluated.