Radioadapted Response Early Effects and Late Effects on Survival of White Blood Cells in Mice (Mus musculus L) Post C0-60 Gamma Radiation

Aims: Determine the radioadapted response to the early and late effects of white blood cells in mice after Co-60 gamma radiation. Furthermore, the effect of the radioadapted early and late effects of the radioresponse with the interval dose adaptation (DA) with the challenge dose (DC) to the white blood cells of mice, as well as the survival rate of mice white blood cells after gamma Co-60 radiation through the radio-adapted response of the early effect and the late effect.

Place and Duration of Study: The Radioteraphy Installation in Prof. I.G.N.G. NGOERAH Hospital, between from August to October 2022.

Methodology: This research used a low-dose method (adapted-DA dose) and at certain time intervals was continued with a larger dose (dose challenge-DC) in mice with six treatments and one as a control. From each treatment, mice's white blood cells were taken to determine the number of leukocytes and their components. Through the number of leukocytes, it is possible to analyze the radio-adaptated response to the early effect and the late effect, so that thesurvival rate of whiteblood cells inmice after gamma Co-60 radiation can be determined.

Results: The results of research on the survival of leukocyte cells, neutrophils, and lymphocyte cell components show early-responding tissue and late-responding tissue. As for cell survival, other componentssuch asmonocytes, eosinophils, andbasophils did not respond in the same way after gamma Co-60 radiation. In general, giving a time delay to the challenge dose for all radiation dose treatments gives a fast response (early effect) to cell survival within a few days after radiation, then with time the full response that occurs is a slow (late effect). The longer the delay in giving the challenge dose, the slower the response will fully occur in the tissue after gamma Co-60 radiation. The cell survival curve also shows that the α/β ratio for the early effect is 3 and for the late effect is 10, which is an illustration of the early effect on radiation, with a linear shape of the cell death curve indicating that tissue is more sensitive to changes in radiation dose and has a greater α/β ratio. When compared to the tissue with a late effect on radiation, the tissue has a smaller α/β ratio indicating that the tissue is less sensitive to changes in the timing of the challenge dose.

Conclusion: Radioadapted response to the survival of leukocytes, neutrophils cell components, and lymphocytes have shown early responding tissue and late responding tissue after gamma Co-60 radiation. By giving a time lag for the challenge dose, an early response to cell survival occurs for only a few days, then with time, fully the response that occurs is late responding.