RP-HPLC Determination of Benzoic Acid in Samples of Children Food Distributed in Aden-Yemen

Reverse phase high-performance liquid chromatographic method that allows the determination of the preservative benzoic acid is described. Separations were affected by using an initial mobile phase of methanol–acetate buffer (pH 4.4), (35:65). Separation of benzoic acid is achieved in 7.10 min. Analytical performance of separation such as the limit of detection, limit of quantification, linear range and reproducibility were evaluated. The method was applied to determine the quantities of benzoic acid in 32 local and imported children food products distributed in Aden-Yemen, comprising, dry milk and chesses, food for infants and young children; cakes, juices and jam. The results show that the ranges of benzoic acid in the studied samples vary greatly from 1.8 mg/L to 849.3 mg/L.