Evaluation of bacterial culture and their resistant pattern in pus containing patients of different wards of the hospital, Lahore, Pakistan

In health care delivery worldwide, infections acquired in health care setting are the most challenging factors. Infections due to antibiotic resistant bacteria have increased day by day in both developed and developing countries. This study is performed to screen the bacterial pathogens present in pus samples taken from patients of different wards of hospital. Isolation of bacteria was determined by standard microbial techniques. Total number of samples is 383 in which 252 were males and 131 were females. 265 samples have growth (259 were suitable for culturing and other 6 were not suitable for culturing) and 118 samples have no growth. Most samples have single growth, while some samples have mixed growth. The predominant genera were coliform including Escherichia coli, Klebsiella, Pseudomonas. Staphylococcus aureus has small number of cultures. These bacterial pathogens show resistant to most of the commonly used antibiotics. The resistant pattern was high in both gram negative and gram positive bacteria. Some organisms show multi-drug resistant pattern.