LOW-COST MEDIA IS AN ALTERNATIVE NOVEL TECHNIQUE TO REGENERATE in vitro Gynura procumbens (Lour.) Merr. INIMITABLE MEDICINAL PLANT

The present research was carried out to develop an alternative in vitro low-cost culture medium for regeneration of an inimitable medicinal plant Gynura procumbens. The nodal segment has been utilized as explant and six separate types of low-cost medium (LCM) which includes LCM-1 (100 mL coconut water); LCM-2 (150 mL coconut water); LCM-3 (200 mL coconut water); LCM-4 (100 mL banana pulp); LCM-5 (150 mL banana pulp); LCM-6, (200 mL banana pulp) and the complete strength MS medium was employed as a control treatment for the standardization of in vitro low-cost regeneration protocol for the Gynura procumbens. All the culture media were supplemented with 2.0 mg / L BA + 0.5 mg / L NAA for shooting and rooting with 0.5 mg / L IBA. Compared to other low-cost culture media, culture media LCM-2 showed the best results in shoot initiation, shoot multiplication, and root formation. As regards cost analysis, LCM-2 appears to minimize cost (78.22%) of the medium per plantlet compared to the regular standard MS medium. Medium of acclimatization consisting of garden soil + Vermicompost + coconut dust (1:1:1, v / v) demonstrated the best hardening medium with maximum survival rate and robust growth performance. Our findings suggest that the LCM-2 culture medium for the regeneration of Gynura procumbens aseptically is an economically cost-efficient protocol and an alternative to the regular standard MS medium.